Table 4

Microarray analysis of selected genes showing differential expression in cepR, cciR or cepRcciIR mutants compared to K56-2.

Gene

Functiona

Change (fold) for K56-R2 (cepR) vs K56-2

Change (fold) for K56-2cciR vs K56-2

Change (fold) for K56-2cepRcciIR vs K56-2


microarrayb

microarrayb

microarrayb


BCAL1369

Sigma factor 70 EcfC (FecI)

4.6

NC

3.0

BCAL1370

Iron uptake regulatory protein FecR

2.7

NC

2.1

BCAL1520

Putative lipoprotein

-2.1

NC

NC

BCAL1528

Flp type pilus assembly protein

-2.1

NC

NC

BCAL1530

Flp type pilus assembly protein

-3.2

NC

-2.0

BCAL1531

Flp type pilus assembly protein

-2.2

NC

NC

BCAL1532

Flp type pilus assembly protein

NC

NC

-2.2

BCAL1533

Putative lipoprotein

-2.1

2.3

NC

BCAL1534

Putative exported protein

-3.3

2.6

NC

BCAL1368

Probable porin

-2.4

2.5

-2.1

BCAL1677

Putative type-1 fimbrial protein

-2.3

3.1

NC

BCAL1688

sigma factor 70 EcfI (OrbS)

NC

-2.5

NC

BCAL1689

MbtH-like protein OrbH

NC

-2.2

NC

BCAL1690

putative dioxygenase OrbG

NC

NC

2.5

BCAL1692

iron transport-related membrane protein OrbD

NC

NC

3.0

BCAL1693

iron transport-related membrane protein OrbF

NC

NC

2.1

BCAL1694

iron transport-related exported protein OrbB

NC

NC

2.4

BCAL1696

ornibactin biosynthesis non-ribosomal peptide synthase OrbI

NC

-2.4

NC

BCAL1697

ornibactin biosynthesis non-ribosomal peptide synthase OrbJ

NC

NC

2.4

BCAL1698

ornibactin biosynthesis protein OrbK

NC

-2.8

NC

BCAL1699

L-ornithine 5-monooxygenase PvdA

NC

-2.8

NC

BCAL1700

ornibactin receptor precursor OrbA

NC

-2.2

2.2

BCAL1701

ornibactin synthetase OrbF

NC

NC

2.2

BCAL1702

ornibactin biosynthesis protein OrbL

NC

NC

2.5

BCAL1722

Putative exported chitinase

-2.8

NC

NC

BCAL2757

Superoxide dismutase SodB

-2.3

NC

NC

BCAL3297

Ferretin DPS-family DNA-binding protein

-2.8

NC

-3.8

BCAL3298

Conserved hypothetical protein

-2.1

NC

-2.8

BCAL3299

Peroxidase/catablase KatB

-2.3

NC

-4.0

BCAM0184

Lectin

NC

2.0

-2.1

BCAM0186

Lectin BclA

-7.5

3.1

-3.2

BCAM0233

ArsR family regulatory protein

NC

-2.0

NC

BCAM0238

Putative ion transporter

NC

2.0

NC

BCAM0239a

N-acylhomoserine lactone synthase CciI

NC

52.1

NC

BCAM0240

N-acylhomoserine lactone dependent regulatory protein CciR

NC

4.0

-23.5

BCAM0949

Exported lipase LipA

-3.1

NC

NC

BCAM0950

Lipase chaperone LipB

-2.5

NC

NC

BCAM1869

Conserved hypothetical protein

-5.9

NC

NC

BCAM1871

Conserved hypothetical protein

-37.6

NC

-20.2

BCAM2307

Zinc metalloprotease ZmpB

-2.3

2.1

-2.4

pBCA055

Putative membrane protein

-12.0

2.4

-9.1


aFunction derived from B. cenocepacia J2315 [34].

bChange in 16-h cultures of cepR, cciR or cepRcciIR mutants compared to 16-h cultures of K56-2 as determined by microarray analysis with at least two biological replicates (NC, no change).

O'Grady et al. BMC Genomics 2009 10:441   doi:10.1186/1471-2164-10-441

Open Data