BMC Genomics

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Open Access Research article

Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa

Mark W Davey1*, Neil S Graham2, Bartel Vanholme3, Rony Swennen4, Sean T May2 and Johan Keulemans1

Author Affiliations

1 Laboratory for Fruit Breeding and Biotechnology, Department of Biosystems, Katholieke Universiteit Leuven, Box 2747, Willem De Croylaan 42, B-3001, Heverlee, Leuven, Belgium

2 Nottingham Arabidopsis Stock Centre, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK

3 Department of Plant Systems Biology, VIB, and Department of Molecular Genetics, Universiteit Gent, Technologiepark 927, B-9052 Gent, Belgium

4 Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 13 Box 2455, B - 3001 Leuven, Belgium

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BMC Genomics 2009, 10:436 doi:10.1186/1471-2164-10-436

Published: 16 September 2009

Additional files

Additional file 1:

Davey, Table 1 - List 2-fold drought-responsive genes. List of genes >2-fold differentially regulated in response to 3-weeks chronic drought stress in Musa variety 'Cachaco', using a gDNA-based hybridisation intensity threshold value (CDF value) of 550.

Format: ZIP Size: 616KB Download file

Open Data

Additional file 2:

Davey, Table 2 - MapMan mappings for ATH drought homologues. List of the MapMan mappings for available Arabidopsis homologues of the list of drought-responsive Musa transcripts.

Format: ZIP Size: 19KB Download file

Open Data

Additional file 3:

Davey, Table 3 - List common drought responsive genes. List of the 330, >2-fold drought-responsive genes common to Musa 3-week chronic drought stress experiment and rice seedling dehydration stress experiment (GEO GSE6901 Rice drought). Differentially expressed Musa transcripts identified using a hybridisation intensity threshold value (CDF value) of 550.

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Open Data

Additional file 4:

Davey, Table 4 - fertigation solution. Table detailing the composition of the fertigation solution used for the growth of plants used in this experiment.

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Additional file 5:

Davey, Table 5 - RT-PCR transcripts and primers. Subset of drought-responsive Musa genes, annotations and relative expression levels as checked by semi-quantitative RT-PCR.

Format: ZIP Size: 13KB Download file

Open Data