Table 8 |
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Combinations of primers used in quantitative PCR assays. |
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|
Gene name |
Gene function |
Primer sequences |
|
|
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|
18S |
Ribosomal protein |
F: CTGGTTAATTCCGATAACGAACGAGACTCTA |
|
R: TGCTCAATCTCGTGTGGCTAAACGCCACTTG |
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|
Hemocyte defensin |
Immune system |
F: ACAAATGTAACAGGCATTGTAGGAGCAT |
|
R: CATGTGCATCTTCGGTAAAAAGTCCA |
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|
Big defensin |
Immune system |
F: ATGGACACTAGGAAAGTCTACTGTGTGCT |
|
R: ACAAGTGCAACCCAGACCCAAGGTGA |
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|
Lysozyme |
Immune system |
F: ATAACGAAAGACCAAGCTCGTGCTCT |
|
R: GTTTTGGGTCCTAGATCTCCCCTGTA |
||
|
C1q – TNF related protein |
Immune system |
F: ATGCAAGTCAGTGCCGTGATACACCCAGA |
|
R: AATAAAGCGCCACTGAAAGTTGTTCCATG |
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|
TRAF-6 |
Immune system |
F: GAACTAGCAAACAGGAATTGGGAGGCGCT |
|
R:GTCAAGTGATGGCTCATCTTGGATGCTGC |
||
|
Toll-like receptor |
Immune system |
F: GTAACAAATTTCACTCTGGCCGCTGACGC |
|
R: TAGCTGAAATCCAACGACTGCACCCGTAA |
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|
RACK-1 |
Cell communication |
F: CCTAACAGATACTGGCTGTGTGCTGC |
|
R: GTCTGTCCATCTGCGGACCATGCAAG |
||
|
Peroxisome proliferator-activated receptor |
Cell communication |
F: CATAGCCAATTCCATACCCCTGGCCA |
|
R: AGTTGGCATCGCCACTGTCGCTGCTC |
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|
HSP70 |
Stress response |
F: AATGACAAAGGCCGTCTCAGCAAGGA |
|
R: TCTAACCAACTGATGACCTCGCTACA |
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|
Stress-induced protein STI |
Stress response |
F: GAAGCTGTTGAACAAGCCAAGAGTGGAGC |
|
R: GTCTCTTGAATTCGGGGATCTTGAGCTGC |
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|
Ferritin |
Iron transport |
F: ATGTCTGTTTCACGACCTCGACAGAA |
|
R: AGTTTCTCGGCATGCTCACGTTCCTC |
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|
Metallothionein |
Detoxification |
F: ACCAGTGATGGTGGCTGCAGGTGTGG |
|
R: TTACACGAACAGCCACTATCACACTG |
||
|
Actin |
Cytoskeleton |
F: ATTGTGATGGACTCTGGTGATGGTGT |
|
R: TCTCTAACAATTTCTCTCTCAGCCGTTGT |
||
|
Transcription factor AP-1 |
Transcription |
F: AGAAAACTTGAAAGAATTGCGCGACT |
|
R: TGTGACATCATTATCTGGCACCCACT |
||
|
Elongation factor beta |
Transcription |
F: CCTTGGGATGATGAAACAGATATGGC |
|
R: CTAATCTTGGCATCTTCTATAACAGC |
||
|
NADH sub-unit IV |
Mitochondrial respiration |
F: CCGTGGGATTTAGGGAGGGATAATATGCT |
|
R: ACTCCAGTTAACAACATTGATCCCCTCAA |
||
|
Senescence associated protein |
unknown |
F: AACCTGTCTCACGACGGTCTAAGCCCAGC |
|
R: TTACCACAGGGATAACTGGCTTGTGG |
||
|
Cytochrome P450 like TBP |
unknown |
F: GTCTGGAAAACGGCCACAAGGCACCT |
|
R: TTATACAAGGTAACCGGCTTGGACGC |
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|
|
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|
Perrigault et al. BMC Genomics 2009 10:377 doi:10.1186/1471-2164-10-377 |
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