a-b. qPCR and exon-level assessment of gene knockdown by siRNA. 3a. Percentage mRNA knock-down 48 hours after siRNA transfection compared to a control siRNA tagetting the control gene Lamp1. 3b. Example of gene knock-down by exon array analysis. Level of knock-down at each of the 23 exon probesets across the entire length of the Nfkb2 transcript in the presence of IFNγ. The green line represents the median signal intensity in the three control arrays (RISC-Free) and red line the median signal intensity in the three Nfkb2 siRNA knock-down arrays (for other exon-level results see Additional file 3).
Lacaze et al. BMC Genomics 2009 10:372 doi:10.1186/1471-2164-10-372