Table 5

Cost per sample and number of mapped reads with increasing multiplexing

Number of barcoded adapters

Fraction of non-barcoded cost per sample*

Number of mapped reads**


1

100%

10.00 M

4

34.61%

2.50 M

8

23.71%

1.25 M

12

20.08%

0.83 M

16

18.26%

0.63 M

40

15.00%

0.25 M


*Includes Illumina DNA library preparation (fixed cost), sequencing run

*Excludes ChIP reagents, Illumina GAII sequencer, HR and data storage

*For a non-barcoded sample, the cost per sample is $498.23 USD as of October 2008.

**Assumes optimal mapping values for a lane run on a GA II (10 million mapped reads)

Lefran├žois et al. BMC Genomics 2009 10:37   doi:10.1186/1471-2164-10-37

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