PolII signal profiles recapitulate findings from Steinmetz et al. PolII ChIP-Seq signal profiles resemble very closely to those published in Figure 3 of Steinmetz et al . We obtained consistent binding at the Bap2-Tat1 loci (a) and at the Sed1-Shu2 loci (b). As expected, we did not observe binding at the Flo11 locus (c). For PolII ChIP-Seq experiments, two biological replicates were barcoded with ACGT (PolII_Rep1, dark blue; PolII_Rep2, orange), one was barcoded with TGCT (PolII_Rep3, red) and a fourth replicate had non-barcoded adapters (PolII_Rep4, green). Input DNA serves as a reference (light blue). Axis and scale normalizations are similar to Figure 2. ORFs above the coordinates axis are on the Watson strand while ORFs below this axis are on the Crick strand.
Lefrançois et al. BMC Genomics 2009 10:37 doi:10.1186/1471-2164-10-37