Figure 4.

Simulation results for combinations of Next Generation sequencing technologies. Illustrating predicted outcomes from combined sequencing technologies with a complex library expressing ca. 18,000 genes. A-C) Combinations include 3.125, 12.5, or 50 MB 5' Sanger sequencing plus 0 to 4 plates of GSFLX and/or Solexa sequence. Each technology or combination of technologies is represented by a different line color, with black indicating Sanger alone, and blue, red, and green lines indicating the addition of GSFLX, Solexa, and GSFLX+Solexa, respectively. The square (n = 1), triangle (n = 2), and diamond (n = 4) shaped points on each line indicate the number of plates added for each technology. Results shown for non-normalized libraries. A) Mean length of longest Unigene per gene (bp), B) Transcriptome Coverage (%), and C) Number of Genes with 100% coverage as a function of total sequence (MB, left panel) and estimated sequencing cost ($1000, right panel) for the different technologies and combinations of technologies. Abbreviations and cost functions are as described in Figures 3D, 3E, and 3F) Combinations include 100, 200, or 400 MB of GSFLX non-normalized or normalized sequencing plus 0 to 4 plates of Solexa sequence. Each technology or combination of technologies is represented by a different line color, with black and red lines indicating GSFLX alone with non-normalized and normalized libraries, respectively. Green and pink lines indicate the addition of Solexa non-normalized and normalized sequences, respectively. The square (n = 1), triangle (n = 2), and diamond (n = 4) shaped points on each line indicate the number of plates added for each technology. D) Mean length of longest Unigene per gene (bp), E) Transcriptome Coverage (%), and F) Number of Genes with 100% coverage as a function of total sequence (MB, left panel) and estimated sequencing cost ($1000, right panel) for the different technologies.