DNA cycle sequencing using an extendable primer. Panel A contains the design of an EOs and TOs for the sequencing of a region of the Escherichia coli streptomycin operon. Vertical bars indicate regions of clamp region of hybridisation between the EO and TO. Capital letters show actual sequence of the oligonucleotides and small, underlined letters indicate the extended region of the EO. N indicates a position with either A, T, G or C and S indicates a position with either G or C nucleotide. Panel B shows an electropherogram of a DNA sequencing reaction using E827 and T827N3. The sequencing reaction was separated and analyzed on an ABI PRISM 377 DNA sequencer and ABI PRISM sequence analysis software.
Cairns et al. BMC Genomics 2009 10:344 doi:10.1186/1471-2164-10-344