Table 1

Lower limits of total RNA required for each amplification method.

Amplification technique Modified T7

Amplification yield, BT474 RNA(mcg)


140 pg

0.80 – insufficient

280 pg

0.60 – insufficient

375 pg

0.16 – insufficient

500 pg

1.4 – sufficient for 1 array

500 pg

1.7 – sufficient for 1 array

500 pg

0 – insufficient

500 pg

0 – insufficient

500 pg

1.9 – sufficient for 1 array

1 ng

17.8

1 ng

20.3

Arcturus

100 pg

0 – insufficient

100 pg

0 – insufficient

250 pg

12

250 pg

7.2

500 pg

56.8

1 ng

52.7

Balanced PCR*

500 pg

0 – insufficient

500 pg

1.5 – sufficient for 1 array

667 pg

2.1 – sufficient for 1 array

667 pg

2.0 – sufficient for 1 array

1 ng

0 – insufficient

1 ng

2.0 – sufficient for 1 array

3.3 ng

2.3 – sufficient for 1 array

3.3 ng

3.2


*1 array discarded due to poor hybridization

Lang et al. BMC Genomics 2009 10:326   doi:10.1186/1471-2164-10-326

Open Data