BMC Genomics

official impact factor 4.21

Open Access Research article

GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation

Murat A Abdurashitov*, Valery A Chernukhin, Danila A Gonchar and Sergey K Degtyarev

Author Affiliations

SibEnzyme, 2/12 Ak. Timakov Str, 630117 Novosibirsk, Russia

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BMC Genomics 2009, 10:322 doi:10.1186/1471-2164-10-322

Published: 17 July 2009

Additional files

Additional file 1:

Alignment of the sequenced GlaI, Sse9I and AspS9I fragments. Numbering is given according to consensus sequence [11]. ACGT sites are given with yellow background. Nucleotides which differ from those in consensus are given with blue background. Sse9I fragments (names start with S) and AspS9I fragments (names start with A) are indicated in green. Some pieces of fragments (shown in magenta) were moved in the alignment to fit to a total length of 234 bp. CG dinucleotides are shown in blue. Yellow background indicates ACGT sites (cleaved or not cleaved).

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Open Data

Additional file 2:

Alignment of 30 γ-satellite MnlI DNA fragments (Vissell and Choo, [13]). Numbering is given according to consensus sequence [11]. ACGT sites are given with yellow background. CG dinucleotides are in blue. Consensus sequence obtained from this alignment is given at the bottom (grey background) and positions which differ from those in a known consensus [11] are indicated by blue background.

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Open Data

Additional file 3:

Alignment of 140 γ-satellite DNA monomers from chromosome 9. Explanations are the same as for Additional file 2.

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Open Data

Additional file 4:

Alignment of 40 γ-satellite DNA fragments from chromosome 3. Explanations are the same as for Additional file 2.

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Open Data