GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation
-
* Corresponding author: Murat A Abdurashitov abd@sibenzyme.ru
SibEnzyme, 2/12 Ak. Timakov Str, 630117 Novosibirsk, Russia
BMC Genomics 2009, 10:322 doi:10.1186/1471-2164-10-322
Published: 17 July 2009Additional files
Additional file 1:
Alignment of the sequenced GlaI, Sse9I and AspS9I fragments. Numbering is given according to consensus sequence [11]. ACGT sites are given with yellow background. Nucleotides which differ from those in consensus are given with blue background. Sse9I fragments (names start with S) and AspS9I fragments (names start with A) are indicated in green. Some pieces of fragments (shown in magenta) were moved in the alignment to fit to a total length of 234 bp. CG dinucleotides are shown in blue. Yellow background indicates ACGT sites (cleaved or not cleaved).
Format: PDF Size: 188KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
Alignment of 30 γ-satellite MnlI DNA fragments (Vissell and Choo, [13]). Numbering is given according to consensus sequence [11]. ACGT sites are given with yellow background. CG dinucleotides are in blue. Consensus sequence obtained from this alignment is given at the bottom (grey background) and positions which differ from those in a known consensus [11] are indicated by blue background.
Format: PDF Size: 334KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
Alignment of 140 γ-satellite DNA monomers from chromosome 9. Explanations are the same as for Additional file 2.
Format: PDF Size: 344KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 4:
Alignment of 40 γ-satellite DNA fragments from chromosome 3. Explanations are the same as for Additional file 2.
Format: PDF Size: 335KB Download file
This file can be viewed with: Adobe Acrobat Reader
