Table 2

Transcripts up and down regulated in Plk4 heterozygous MEFs.

Upregulated Genes

Cell Cycle

Gene ID

Fold Change

Putative p53 site

Metabolism

Gene ID

Fold Change

Putative p53 site


Casein kinase II (Csnk2a1)

12995

5.8040

20

N-acylsphingosine amidohydrolase (acid ceramidase) like (Asahl)

11886

6.0539

14

Protein phosphatase 1F (PP2C domain containing) (Ppm1f)

68606

4.8644

10

Leucyl/cystinyl aminopeptidase (Lnpep)

240028

4.8539

6

Squamous cell carcinoma antigen recognized by T-cells 1 (Sart1)

20227

4.6888

20

Galactose-4-epimerase (Gale)

74246

4.7700

8

Origin recognition complex, subunit 4-like (Orc4l)

26428

4.5661

9

L-2-hydroxyglutarate dehydrogenase (L2hgdh)

217666

4.6257

15

Inhibitor of DNA binding 2 (Id2)

15902

4.4323

11

Fatty acid desaturase 3 (Fads3)

286922

4.2976

27

Protein phosphatase 5 (Ppp5c)

65179

4.0765

15

Carbohydrate sulfotransferase 2 (Chst2)

54371

4.2926

18

heme binding protein 2 (Hebp2)

56016

3.8955

10

Stearoyl-Coenzyme A desaturase 1 (Ankrd13c)

433667

3.9806

16

Neuropilin (Nrp1)

18186

3.0956

13

CCR4 carbon catabolite repression like 4 (Ccrn4l)

310395

3.7939

14

Prohibitin (Phb)

18673

3.0459

18

protein kinase, cAMP dependent regulatory, type I beta (Prkar1b)

19085

3.3199

18

Cyclin dependent kinase 8 (Cdk8)

264064

3.0094

17

DNA Repair

Heterogeneous nuclear ribonucleoprotein C (Hnrpc)

15381

2.5962

8

Uracil-DNA glycosylase (Ung)

22256

4.1846

15

TVMSFG fibroblast growth factor receptor 1 precursor (Fgfr1)

14182

2.2643

18

MutS homolog 6 (Msh6)

17688

2.1811

23

Phosphatidylinositol 3-kinase (Pic3c2a)

18704

2.1636

9

Thymine DNA glycosylase (Tdg)

21665

2.0156

10

Pituitary tumor-transforming 1 (Pttg1)

30939

1.9518

11

Development

Transcriptional/Translational Regulation

Sal-like 3 (Sall3)

20689

7.9335

13

Cysteinyl-tRNA synthetase (Cars)

27267

4.8072

18

T-cell factor 4 (Rab27b)

80718

4.7804

14

Zinc Finger Protein 451 (Zfp451)

98403

4.1537

9

Nuclear receptor co-repressor 1 (Ncor1)

20185

4.4658

16

Tetratricopeptide repeat domain 1 (Ttc1)

66827

4.0003

20

Inositol 1,4,5-triphosphate receptor 5 (Itpr2)

16439

3.6909

10

Highly similar to CBP_MOUSE CREB-binding protein (Crebbp)

12914

3.7483

9

Procollagen, type VI, alpha 3 (Col6a3)

12835

3.5828

15

Zinc finger protein 689 (Zfp689)

71131

3.4636

17

Fetal Alzheimer antigen (Bptf)

207165

3.3594

NA

Glutamyl-prolyl-tRNA synthetase (Eprs)

107508

3.3065

19

WNT1 inducible signaling pathway protein 1 (Wisp1)

22402

3.2886

22

GC-rich sequence DNA-binding factor homolog isoform 1 (C21orf66)

67367

3.0984

N/A

T-box transcription factor Tbx15 (Tbx15)

21384

2.9248

13

Phenylalanine-tRNA synthetase 2 (Fars2)

69955

3.0678

13

Nuclear factor I/X (Nfix)

18032

2.8883

9

GLIS family zinc finger 3 (Glis3)

226075

2.6233

10

Thrombospondin 2 (Thbs2)

21826

2.5437

23

Transmembrane and tetratricopeptide repeat containing 2 (Tmtc2)

278279

2.2096

18

Osteopontin (Spp1)

20750

2.4717

15

Transcription factor A (Tfam)

21780

2.0680

14

Fukuyama type congenital muscular dystrophy homolog (Fktn)

246179

2.2362

9

DNA Methylation

Cellular/Ion Transport

SAP30 binding protein (Sap30bp)

57230

2.9834

8

Pleckstrin (Plek)

56193

6.0575

11

SET domain ERG-associated histone methyltransferase (Olfml3)

99543

2.0889

9

Syntaxin 18 (Stx18)

53407

5.0970

18

Calcium binding and coiled coil domain 1 (Calcoco1)

67488

5.6489

19

Aquaporin-1 (Aqp1)

11826

4.4128

16

Solute carrier family 6 (Slc6a6)

21366

4.4110

18

Miscellaneous Cellular Functions

Exocyst complex component 3 (Exoc3)

211446

3.9296

10

Coiled Coil domain containing 131 (Ccdc131)

216345

6.4372

11

Protein-coupled receptor 19 (Gpr19)

14760

3.8281

21

Thyroid hormone receptor interactor 11 (Trip11)

109181

5.8691

NA

Solute carrier family 39 (Slc39a10)

227059

3.5028

13

Smg-6 homolog (Smg6)

103677

5.3375

11

Frequenin homolog (Freq)

14299

3.1881

15

Talin 2 (Tln2)

70549

5.2780

N/A

Serine Hydrolase like (Serhl)

68607

3.1718

17

Tomoregulin 1 (Tmeff1)

230157

5.1116

7

Solute carrier family 14 (Slc14a2)

27411

3.0842

14

Channel-interacting PDZ domain protein (Inadl)

12695

4.9504

14

Translocator of inner mitochondrial membrane (Timm17b)

21855

2.7129

19

WD repeat domain 50 (Utp18)

217109

4.9252

16

Similar to crooked neck protein (Ipo7)

233726

2.5873

17

Inositol hexaphosphate kinase 1 (Ip6k1)

27399

4.8086

12

Oxysterol binding protein like protein 9 (Osbpl9)

100273

2.5501

12

Spetex-2E protein (100040875)

4.6574

N/A

ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2)

11941

2.4328

26

Multiple PDZ domain protein (Mpdz)

17475

4.0596

12

Transient receptor potential cation channel, subfamily M, member 7 (Trpm7) 58800

1.9917

11

Myosin heavy chain 10 (Myh10)

77579

3.8880

11

CDC42 effector protein (Rho GTPase binding) 2 (Cdc42ep2)

104252

3.5581

23

Downregulated Genes

Zinc finger protein 507 (Zfp507)

668501

3.4688

N/A

Development

aarF domain containing kinase 1 (Adck1)

72113

3.4641

9

Procollagen, type III, alpha 1 (Col3a1)

12825

-4.6364

6

AHNAK nucleoprotein (Ahnak)

66395

3.2688

20

Procollagen, type V, alpha 2 (Col5a2)

12832

-4.1420

12

Ring finger protein 11 (Arhgdia)

192662

3.1199

25

Oral-facial-digital syndrome 1 gene homolog (Ofd1)

237222

-3.0782

8

Villin (Vil1)

22349

2.9808

15

Procollagen, type I, alpha 2 (Col1a2)

12843

-2.9648

12

3-phosphoglycerate dehydrogenase (Phgdh)

236539

2.9427

18

Metabolism

Arginine/serine-rich coiled-coil 1 (Rsrc1)

66880

2.9404

6

Stearoyl-Coenzyme A desaturase 2 (Scd2)

20250

-2.4729

10

Olfactory receptor 202 (Olfr202)

258997

2.9278

7

Mus musculus mVL30-1 retroelement mRNA sequence (mVL30-1)

-2.5566

N/A

Discs, large homolog 5 (Dlg5)

71228

2.9094

N/A

Transmembrane protein 34 (Tmem184c)

234463

-2.5241

14

2'-phosphodiesterase (E430028B21Rik)

2.8426

0

Mus musculus 0 day neonate cerebellum cDNA (E430024C06Rik)

319443

-2.3000

N/A

HD domain containing 3 (Hdcc3)

68695

2.7345

14

Hypothetical protein LOC639390 (LOC639390)

-2.2121

N/A

Heat shock protein 1(Heatr1)

217995

2.7243

13

Myotubularin related protein 7 (Mtmr7)

54384

2.4370

8

Mitochondrial ribosomal protein L50 (Mrpl50)

362517

2.2540

20

Proteasome(macropain)26S subunit, non-ATPase (Psmd4)

19185

2.2031

11

NICE-5 protein (AA414768)

245350

2.1272

N/A


Shown above are ninety-seven upregulated genes and nine downregulated genes in the heterozygous MEFs. "Fold change" is the change in transcript level in the heterozygous MEFs relative to wild-type MEFs as determined by microarray analysis. The "Putative p53 sites" are as determined by MAPPER and is based on the first 5000 nucleotides upstream of the transcriptional start site. Please note that forty-six downregulated transcripts and their corresponding gene were omitted from the table as they encode hypothetical proteins or have unknown cellular functions. All the microarray data has been deposited into the MIAME database under accession number E-MEXP-2161.

Morettin et al. BMC Genomics 2009 10:319   doi:10.1186/1471-2164-10-319

Open Data