p53 activity and model of Plk4 and p53 interactions in Plk4 heterozygous MEFs. A.) Effect of Plk4 haploinsufficiency on p53 activity. p53 transcriptional activity was analyzed in Plk4 heterozygous and wild-type MEFs from nuclear extracts using a capture ELISA assay. The p53 specificity was confirmed upon incubation with competing labeled and unlabeled oligonucleotides. The results were normalized against wild-type control and are representative of three independent experiments. B.) Model of p53 and Plk4 regulation in Plk4 heterozygous and wild-type MEFs. Shown are proposed Plk4/p53 direct/indirect interactions in Plk4 wild-type and heterozygous MEFs with the resultant corresponding changes in protein levels, activity and gene expression. Solid lines indicate pathways that are functionally active, whereas dotted lines indicate pathways with reduced activity.
Morettin et al. BMC Genomics 2009 10:319 doi:10.1186/1471-2164-10-319