Methodology article
Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing
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* Corresponding author: Alfredo Herrera-Estrella aherrera@ira.cinvestav.mx
1 Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km 9.6 Libramiento Norte, carretera Irapuato-León. 36821, Irapuato, Gto, Mexico
2 Boyce Thompson Institute for Plant Research, Ithaca NY, 14853, USA
BMC Genomics 2009, 10:299 doi:10.1186/1471-2164-10-299
Published: 6 July 2009Additional files
Additional file 1:
A histogram showing the linear increase of the number of sequences after four GS20–454 runs. The number of high quality sequences is plotted with the number of runs. The total of the sequences generated by all the four sequencing runs are depicted.
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Additional file 2:
Number of matching NCBI maize ESTs and MAGIs to the Palomero GS20–454 sequences after each sequencing run. This table summarizes the BLAST results of all the Palomero GS20–454 reads against the NCBI ESTs and MAGIs.
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Additional file 3:
Primers used in this study. This table contains the sequence of primers used for qRT-PCR.
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