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Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH)

Roy R Chaudhuri1, Andrew G Allen24, Paul J Owen25, Gil Shalom26, Karl Stone27, Marcus Harrison27, Timothy A Burgis28, Michael Lockyer2, Jorge Garcia-Lara3, Simon J Foster3, Stephen J Pleasance1, Sarah E Peters1, Duncan J Maskell1 and Ian G Charles3*

Author Affiliations

1 Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK

2 Arrow Therapeutics, Britannia House, 7 Trinity Street, London, SE1 1DB, UK

3 Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK

4 Current address: Pfizer Australia, Veterinary Medicine Research and Development, 45 Poplar Road, Parkville, 3052 Victoria, Australia

5 Current address: Cancer Research Technology Development Laboratory, Wolfson Institute for Biomedical Research, The Cruciform Building, Gower Street, London, WC1E 6BT, UK

6 Current address: Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WC1X 8LD, UK

7 Current address: Oxford Gene Technology, Begbroke Science Park, Sandy Lane, Yarnton, Oxford, OX5 1PF, UK

8 Current address: Centre for Bioinformatics, Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London, SW7 2AZ, UK

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BMC Genomics 2009, 10:291  doi:10.1186/1471-2164-10-291

Published: 1 July 2009

Additional files

Additional file 1:

Essential S. aureus genes, and comparison with the essential gene complement of B. subtilis and previous S. aureus essential gene studies. A comprehensive list of S. aureus essential genes identified in this study. The names Bae, Ko, Forsyth and Ji refer to the primary authors of previously published S. aureus essential gene studies[13,19-21]. The B. subtilis essential gene data is derived from the study of Kobayashi et al. [4].

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Additional file 2:

Essential S. aureus genes, and comparison with the essential gene complement of B. subtilis. A compact version of the data presented in Additional file 1, suitable for printing.

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Additional file 3:

Results of PCR/sequencing analysis and linker PCR. Full results from the PCR/sequencing analysis used to validate the microarray screen. Column C indicates the position of the PCR primer relative to the start codon of the target gene. Columns F and G indicate the size range of the PCR products that correspond to an intragenic transposon. Column K indicates the location of the insertion as determined from the sequence data (where available). For intragenic inserts the first number is the position of the insert relative to the start of the gene. LPCR indicates that no PCR products could be obtained, but the gene-specific primer was verified by linker PCR.

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