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Expressed sequence tags from larval gut of the European corn borer (Ostrinia nubilalis): Exploring candidate genes potentially involved in Bacillus thuringiensis toxicity and resistance

Chitvan Khajuria1 email, Yu Cheng Zhu2 email, Ming-Shun Chen1,3 email, Lawrent L Buschman1 email, Randall A Higgins1^ email, Jianxiu Yao1 email, Andre LB Crespo4 email, Blair D Siegfried4 email, Subbaratnam Muthukrishnan5 email and Kun Yan Zhu1 email

Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA

Southern Insect Management Unit, USDA-ARS, 141 Experiment Station Road, Stoneville, MS 38776, USA

Plant Science and Entomology Research Unit, USDA-ARS, Manhattan, KS 66506, USA

Department of Entomology, 202 Plant Industry Building, University of Nebraska, Lincoln, NE 68583, USA

Department of Biochemistry, 141 Chalmers Hall, Kansas State University, Manhattan, KS 66506, USA

author email corresponding author email^Deceased

BMC Genomics 2009, 10:286doi:10.1186/1471-2164-10-286

Published: 29 June 2009

Additional files

Additional file 1:

Sequences of PCR primers used to compare the gene expression profiles of trypsin-like and chymotrypsin-like serine proteases, alkaline phosphatases, aminopeptidases, and cadherin-like protein by RT-PCR between Cry1Ab-susceptible and resistant strains of European corn borer (Ostrinia nubilalis). The information provided represents the sequences of forward and reverse PCR primers, and expected size of PCR product for each of 41 candidate genes.

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