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Expressed sequence tags from larval gut of the European corn borer (Ostrinia nubilalis): Exploring candidate genes potentially involved in Bacillus thuringiensis toxicity and resistance

Chitvan Khajuria1, Yu Cheng Zhu2, Ming-Shun Chen13, Lawrent L Buschman1, Randall A Higgins1, Jianxiu Yao1, Andre LB Crespo4, Blair D Siegfried4, Subbaratnam Muthukrishnan5 and Kun Yan Zhu1*

Author Affiliations

1 Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA

2 Southern Insect Management Unit, USDA-ARS, 141 Experiment Station Road, Stoneville, MS 38776, USA

3 Plant Science and Entomology Research Unit, USDA-ARS, Manhattan, KS 66506, USA

4 Department of Entomology, 202 Plant Industry Building, University of Nebraska, Lincoln, NE 68583, USA

5 Department of Biochemistry, 141 Chalmers Hall, Kansas State University, Manhattan, KS 66506, USA

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BMC Genomics 2009, 10:286  doi:10.1186/1471-2164-10-286

Published: 29 June 2009

Additional files

Additional file 1:

Sequences of PCR primers used to compare the gene expression profiles of trypsin-like and chymotrypsin-like serine proteases, alkaline phosphatases, aminopeptidases, and cadherin-like protein by RT-PCR between Cry1Ab-susceptible and resistant strains of European corn borer (Ostrinia nubilalis). The information provided represents the sequences of forward and reverse PCR primers, and expected size of PCR product for each of 41 candidate genes.

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