Table 4 |
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|
Q-PCR primer pairs used to amplify selected barley and wheat transcripts. |
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|
Probeset |
Forward primer |
Reverse primer |
Product size (bp) |
|
|
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|
Barley1 |
|||
|
Contig8230 |
TACATGCTCTTGTTTGGTGCTACTG |
AAGGTAAGTAGGCAGCAGTGAAGGT |
204 |
|
Contig6943 |
GGGGAAATCCCAGGTCGTCGAT |
GGCTTGCTGCTAGGGTTTTCAG |
266 |
|
Contig7925 |
CGAACCGTAGAATGTGTAAGGG |
GGGAGGAAAGATACACGCTT |
114 |
|
Contig3031 |
TTACTATGCTGGATATGGACAAGGG |
TCTCATCTCATGTCTGGAAGACCC |
190 |
|
Contig4668 |
CCCCCCACAAGTACCTGAAGA |
CGTTGGCTTGCTTAGCTCTTCC |
286 |
|
Contig7671 |
CTAAGCGACCTTGCATCTTTTGAC |
AACGCTAGTGCTACTGGCAGGA |
213 |
|
Contig20269 |
GAAGGCTCAGAAAGTTGCTGCTAT |
GCAAAATCATTCACTGCTTCCAGAG |
224 |
|
Contig5740 |
GAGGCTGTTCAGCAACTGGACTG |
CAAGGATCCCAGCCACATACTG |
227 |
|
Contig15148 |
GATCTCTTCGTGGTGGATCACATAC |
GCTTGATGTCCTATGCTTTCCAA |
221 |
|
Contig11660 |
ACCTCATCAACCTCTGCGGC |
TTCCAGAGAACGGAGGCAGG |
210 |
|
Contig14399 |
AGAAAGAGAGATTTTGAAGCTTGGC |
AATCCATCGCCATGCCAACT |
213 |
|
Contig15147 |
GGCGGGGCACTTTTGAGGACAT |
CGAGCCTGCGACGGGTTATT |
182 |
|
Contig2400 |
AAGCATGCCGCCATCCCGTT |
CCCAACCTGACAACTCCACCTAGA |
244 |
|
Wheat |
|||
|
Ta.3039.1 |
ACGTCCATAACGATGGTCTTCATTG |
GTAGTGGCCTCAGCATCACCATTGC |
170 |
|
Ta.13729.1 |
TTTTCTACATGCTCTTGTTTGGTGC |
AAAAGATCAACCCATGTGCTGCTCC |
265 |
|
Ta.27013.1 |
CGAAGCGTGTATCTTTCCTC |
CAGACACAAACGAAAATGAC |
183 |
|
Ta.7602.1 |
AGCCCCCCACAAGTACCTGATGATG |
GTCGTCATCCTCGTCACCATCTTCC |
201 |
|
Ta.27369.1 |
TTACTATGCTGGATATGGACAAGGC |
TGCTACAACATTAGCCTTGACAGTG |
230 |
|
Ta.9536.1 |
GCCCTAAACGACCTTGCATCTTTTG |
AAACTGAAGCACTAACCTACGACGC |
236 |
|
Ta.968.1 |
ATGTGCTGCGTCGTCAGATACATAG |
TACCCTCCTCGACTTCCTTGTGATC |
204 |
|
Ta.4425.1 |
GATGCCATCAGATCCTCCAATT |
GCCACTCCGTTGTGTCATAATATGG |
235 |
|
Ta.27038.1 |
CGAAGCGTGTATCTTTCCTC |
CAGACACAAACGAAAATGAC |
151 |
|
Ta.7256.1 |
CATCTCATGGTACCTGACTGTCGA |
GCAACAGACTGCCACCAGCA |
264 |
|
TaAffx.46790.1 |
TCATGTCAGTTTATTGCAAGG |
CAGTGACACTATAACAATACAGTTCT |
240 |
|
TaAffx.128707.1 |
GAAAAGGTTGTAGTTCAGAAGG |
TTGCTCTGGACTACTGTCTTC |
259 |
|
|
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|
Primer sequence details used in the Q-PCR verification of selected barley and wheat expression profiles and the size of the corresponding RNA products. |
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|
Schreiber et al. BMC Genomics 2009 10:285 doi:10.1186/1471-2164-10-285 |
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