Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Research article

Gene expression profiling within the spleen of Clostridium perfringens-challenged Broilers fed antibiotic-medicated and non-medicated diets

Aimie J Sarson1, Ying Wang2, Zhumei Kang1, Scot E Dowd3, Yang Lu1, Hai Yu1, Yanming Han4, Huaijun Zhou2 and Joshua Gong1*

  • * Corresponding author: Joshua Gong gongj@agr.gc.ca

  • † Equal contributors

Author Affiliations

1 Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Canada

2 Department of Poultry Science, Texas A &M University, College Station, USA

3 Research and Testing Laboratories and Medical Biofilm Research Institute, Lubbock, USA

4 Nutreco Canada Agresearch, Guelph, Canada

For all author emails, please log on.

BMC Genomics 2009, 10:260  doi:10.1186/1471-2164-10-260

Published: 7 June 2009

Abstract

Background

Clostridium perfringens (Cp) is a Gram-positive anaerobic bacterium that causes necrotic enteritis (NE) in poultry when it overgrows in the small intestine. NE disease has previously been controlled through the use of growth-promoting antibiotics. This practice was recently banned in European countries, leading to significantly increased incidence of NE threatening the poultry industry. Control strategies and technology as substitutes to dietary antibiotics are therefore urgently required. To develop the substitutes, it is important to understand host immune responses to Cp infection. However, the knowledge is still lacking. We therefore investigated gene expression profiles within immunologically-relevant tissue, the spleen, in order to identify factors that are involved in immunity to NE and have potential as therapeutic targets.

Results

Use of a 44 K Agilent chicken genome microarray revealed significant up-regulation of many immune-associated genes in Cp-challenged chickens, including galectin 3, IFNAR1, IgY-receptor, TCR╬│, granzyme A, and mannose-6-P-R, which were subsequently validated by quantitative PCR assays. Functional annotation of differentially expressed genes was conducted using the High Throughput Gene Ontology Functional Annotation database. Medicated and Non-medicated chickens had similar annotation profiles with cell activities and regulation being the most dominant biological processes following Cp infection.

Conclusion

Broiler chickens demonstrated an intricate and holistic magnitude of host response to Cp challenge and the development of NE. Although the influence of dietary antibiotics appeared to be less significant than the disease process, both had a considerable impact on the host response. Markers previously identified in intestinal inflammatory diseases of other species, including humans, and indicators of enhanced antibody responses, appeared to be involved in the chicken response to Cp challenge. The significance in host immune responses of immune mediators identified from the present study warrants further studies to verify their functions during NE development and to determine their potential application to control NE disease.