Open Access Research article

Probing the endosperm gene expression landscape in Brassica napus

Yi Huang1, Liang Chen12, Liping Wang1, Kannan Vijayan1, Sieu Phan3, Ziying Liu3, Lianglu Wan1, Andrew Ross1, Daoquan Xiang1, Raju Datla1, Youlian Pan3* and Jitao Zou1*

Author Affiliations

1 Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

2 School of Life Sciences, Xiamen University, 422 Siming Nan Road, Xiamen, Fujian, 361005, PR China

3 Institute for Information Technology, National Research Council Canada, Ottawa, ON, K1A 0R6, Canada

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BMC Genomics 2009, 10:256  doi:10.1186/1471-2164-10-256

Published: 2 June 2009

Additional files

Additional file 1:

A list of all unisequences identified and their best BLASTX match annotations. 9963 unisequences from endosperm cDNA library with best BLASTX match annotations, unisequence length, contig size, code in dbEST and locus ID.

Format: XLSX Size: 1MB Download file

Open Data

Additional file 2:

Highly abundant unisequences in Brassica napus endosperm. A list of contigs derived from 10 or more ESTs.

Format: XLSX Size: 16KB Download file

Open Data

Additional file 3:

A table of proteins identified in endosperm via protein profiling. A total of 809 genes identified from endosperm protein dataset.

Format: XLSX Size: 64KB Download file

Open Data

Additional file 4:

A table of 1229 significantly differentially expressed unisequences during endosperm development. This table shows annotations and expression level changes of 1229 significantly differentially expressed unisequences during endosperm development.

Format: XLSX Size: 264KB Download file

Open Data

Additional file 5:

A table listing 24 patterns of significantly differentially expressed unisequences during endosperm development. The data show the significantly differentially expressed unisequences list and gene expression level changes in each cluster.

Format: XLSX Size: 345KB Download file

Open Data

Additional file 6:

Biological functional annotation of differentially expressed unisequences in each cluster. The data show the distribution of GO annotation in biological processes for each cluster.

Format: XLSX Size: 10KB Download file

Open Data

Additional file 7:

Tables listing stage-favored genes during endosperm development. These data show stage-favored genes with expression level changes, EST accession numbers in dbEST of NCBI and annotations.

Format: XLSX Size: 279KB Download file

Open Data

Additional file 8:

A table listing universally expressed genes during endosperm development. The table shows universally expressed genes with expression level changes, EST accession numbers in dbEST of NCBI and annotations.

Format: XLSX Size: 134KB Download file

Open Data

Additional file 9:

A table of enzyme-encoding genes identified in the endosperm. Enzyme-encoding genes indentified in Brassica endosperm using cDNA library, protein profiling and cDNA microarray.

Format: XLSX Size: 115KB Download file

Open Data

Additional file 10:

A table of transcription factors identified in the endosperm cDNA library. Unisequences encoding transcription factors in the endosperm identified from cDNA library.

Format: XLSX Size: 51KB Download file

Open Data

Additional file 11:

A file describing methods for microarray data analysis. Details for microarray data analysis

Format: DOCX Size: 25KB Download file

Open Data

Additional file 12:

A table listing primers for RT-PCR validation of the selected unisequences. Primers for RT-PCR validation of the representative unisequences identified by cDNA microarray.

Format: DOCX Size: 13KB Download file

Open Data