Research article
Transcriptomics reveals extensive inducible biotransformation in the soil-dwelling invertebrate Folsomia candida exposed to phenanthrene
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* Corresponding author: Benjamin Nota benjamin.nota@gmail.com
1 VU University Amsterdam, Institute of Ecological Science, Department of Animal Ecology, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands
2 VU University Medical Center, Department of Pathology, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands
BMC Genomics 2009, 10:236 doi:10.1186/1471-2164-10-236
Published: 20 May 2009Additional files
Additional File 1:
Figure S1: Venn diagram of significantly differentially expressed transcripts in response to phenanthrene. In this diagram the amount of differential genes is shown for each phenanthrene concentration within the circles. The number in the right-bottom is the amount of non-significantly expressed genes.
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Additional File 2:
Table S1: All significantly differentially expressed transcripts in response to low concentration (24.95 mg kg-1) phenanthrene in soil. This table contains all significantly differentially expressed transcripts and their log2 transformed fold change values.
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Additional File 3:
Table S2: All significantly differentially expressed transcripts in response to high concentration (45.80 mg kg-1) phenanthrene in soil. This table contains all significantly differentially expressed transcripts and their log2 transformed fold change values.
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Additional File 4:
Figure S2: Heatmap for transcripts differentially expressed in response to the high concentration of phenanthrene (45.80 mg kg-1) and cadmium (57.9 mg kg-1) in soil. Both concentrations represent the EC50 on reproduction after 28 days. Data of 4 microarrays were used for each xenobiotic exposure. Hierarchical clustering of log2 fold change values (treatment/reference) using Euclidean distance matrix, and average linkage. Red indicates upregulation and green downregulation compared to the reference control, black indicates no difference. The transcripts are named by their gene cluster in Collembase, followed by their putative function.
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Additional File 5:
Table S3: Oligos used in the qPCR analysis. All sequences of the primers used in this study are shown, including PCR efficiency values.
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