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Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays

Joshua S Bloom* email, Zia Khan* email, Leonid Kruglyak email, Mona Singh email and Amy A Caudy email

BMC Genomics 2009, 10:221doi:10.1186/1471-2164-10-221

fisher's exact test for differential expression analysis

Ann Loraine   (28 May 2009)  UNC Charlotte email

I'm curious about the authors' use of Fisher's exact test of contingency tables for the short-reads differential expression testing. It's an easy test to perform and it would be great if it's appropriate to use in this setting. But I think the contingency tables, if I understand the approach, will have vastly different numbers in the cells of the table. How does the test perform under these conditions? I would welcome some additional discussion of this issue.

Competing interests

No competing interests.

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