Research article
Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGE
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* Corresponding author: Dave Hansen dhansen@ucalgary.ca
1 Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
2 Canada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 4S6, Canada
3 Department of Mathematics and Statistics, University of Calgary, Calgary, Alberta T2N 1N4, Canada
4 National Institute of Genetics, 1111 Yata, Mishima 411-8540, Japan
5 Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
BMC Genomics 2009, 10:213 doi:10.1186/1471-2164-10-213
Published: 9 May 2009Additional files
Additional File 1:
All of the genes identified by germline SAGE library. A list of genes that were identified by the germline SAGE library used for analysis.
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Additional File 2:
Set of 157 highly expressed genes identified in the germline library. A list of genes, organized according to KOG classification, that have a tag count >60 in the germline SAGE library.
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Additional File 3:
Proteins with putative RNA binding activity. A list of genes that were identified to encode proteins with putative RNA binding activity.
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Additional File 4:
Tag distribution of the RNA binding proteins identified in the germline and soma SAGE libraries. Tag distribution of the RNA binding proteins identified in the germline and soma SAGE libraries. Proteins with potential RNA binding activity were identified as described in Methods. In total, 319 proteins were identified (Additional file 3), with 190 genes present in the germline SAGE library and 131 genes present in the soma SAGE library. Plotted is the total number of genes, of the 190 genes in the germ line and 131 genes in the soma, that have a given tag distribution. The number of genes in each tag range was determined and plotted against the tag distribution.
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Additional File 5:
The 1063 germline-specific/enriched genes identified by SAGE. A list of genes that are identified to be germline-specific/enriched by comparing germline SAGE data with soma SAGE data.
Format: XLS Size: 198KB Download file
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Additional File 6:
Set of 603 germline-specific/enriched genes identified only by SAGE. A list of genes that are germline-specific/enriched genes identified only by SAGE as compared to microarray.
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Additional File 7:
Germline-specific/enriched genes identified only by SAGE with microarray F.I. < 2. Summary of in situ hybridization expression pattern for those genes that are identified only by SAGE to be germline-specific/enriched and have a microarray F.I. < 2.
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