Figure 6.

Codon usage in T. thermophila P450 genes. A: Neutrality plots (GC12 vs. GC3s). The regression line: y = 0.1435x + 0.2853, r2 = 0.1877, OP (optimal point) = 0.333. OP indicates the point at which the regression line crossed the diagonal line. B: The Effective number of codons (ENc) plotted with the GC3s for each predicted P450 gene. Each expected ENc from GC3s is shown as a standard curve. C: Correspondence analysis of the relative synonymous codon usage (RSCU) values. A plot of the two most important axes after the correspondence analysis was shown. The three P450 isoforms (CYP5008A1, CYP5010B1 and CYP5013D1) that each has a CAI value above 0.6 were indicated with brown, orange and red color dot, respectively. The rest of the T. thermophila P450 genes were shown with a blue dot.