Figure 5.

Localization of Bg30001 and Bg30017 expression. In situ hybridization using antisense Bg30001 and Bg30017 RNA probes. mRNA of Bg30001 (A) and Bg30017 (E) is detected in the follicular epithelium of basal oocytes from 7-day-old females in the period of chorion formation, but not in sub-basal oocytes or in the germarium. Detail of the follicular cells showing that Bg30001 (C) and Bg30017 (G) transcripts localize in the cytoplasm of the follicular cells. Control ovarioles tested with sense probes of Bg30001 (B, D) and Bg30017 (F, H) were not labelled. Scale bars: 200 μm in panels A, B, E, F; and 50 μm in panels C, D, G, H.