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Open Access Research article

Mapping QTL affecting resistance to Marek's disease in an F6 advanced intercross population of commercial layer chickens

Eliyahu M Heifetz12*, Janet E Fulton3, Neil P O'Sullivan3, James A Arthur3, Hans Cheng4, Jing Wang15, Morris Soller6 and Jack CM Dekkers1

Author Affiliations

1 Department of Animal Science and Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011, USA

2 Department of Molecular Biology, Ariel University, Ariel 44837, Israel

3 Hy-Line International, Dallas Center, IA 50063, USA

4 USDA-ARS-ADOL, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA

5 Pioneer Hi-Bred International Inc., Johnston, IA 50131, USA

6 Department of Genetics, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

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BMC Genomics 2009, 10:20  doi:10.1186/1471-2164-10-20

Published: 14 January 2009

Abstract

Background

Marek's disease (MD) is a T-cell lymphoma of chickens caused by the Marek's disease virus (MDV), an oncogenic avian herpesvirus. MD is a major cause of economic loss to the poultry industry and the most serious and persistent infectious disease concern. A full-sib intercross population, consisting of five independent families was generated by crossing and repeated intercrossing of two partially inbred commercial White Leghorn layer lines known to differ in genetic resistance to MD. At the F6 generation, a total of 1615 chicks were produced (98 to 248 per family) and phenotyped for MD resistance measured as survival time in days after challenge with a very virulent plus (vv+) strain of MDV.

Results

QTL affecting MD resistance were identified by selective DNA pooling using a panel of 15 SNPs and 217 microsatellite markers. Since MHC blood type (BT) is known to affect MD resistance, a total of 18 independent pool pairs were constructed according to family × BT combination, with some combinations represented twice for technical reasons. Twenty-one QTL regions (QTLR) affecting post-challenge survival time were identified, distributed among 11 chromosomes (GGA1, 2, 3, 4, 5, 8, 9, 15, 18, 26 and Z), with about two-thirds of the MD resistance alleles derived from the more MD resistant parental line. Eight of the QTLR associated with MD resistance, were previously identified in a backcross (BC) mapping study with the same parental lines. Of these, 7 originated from the more resistant line, and one from the less resistant line.

Conclusion

There was considerable evidence suggesting that MD resistance alleles tend to be recessive. The width of the QTLR for these QTL appeared to be reduced about two-fold in the F6 as compared to that found in the previous BC study. These results provide a firm basis for high-resolution linkage disequilibrium mapping and positional cloning of the resistance genes.