Parallel DNA pyrosequencing unveils new zebrafish microRNAs

doi:10.1186/1471-2164-10-195

BMC Genomics

Volume 10

Viewing options:

Abstract
Full text
PDF (1.2MB)
Additional files

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Soares AR
Pereira PM
Santos B
Egas C
Gomes AC
Arrais J
Oliveira JL
Moura GR
Santos MA

on PubMed

Soares AR
Pereira PM
Santos B
Egas C
Gomes AC
Arrais J
Oliveira JL
Moura GR
Santos MA
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Nominate for award

Post to:

Research article

Parallel DNA pyrosequencing unveils new zebrafish microRNAs

Ana R Soares¹^,2 , Patrícia M Pereira¹ , Bruno Santos³ , Conceição Egas²^,3 , Ana C Gomes³ , Joel Arrais⁴ , José L Oliveira⁴ , Gabriela R Moura¹ and Manuel AS Santos¹

¹RNA Biology Laboratory, Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal

²Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal

³Biocant Research Centre, 3060-197 Cantanhede, Portugal

⁴IEETA, University of Aveiro, 3810-193 Aveiro, Portugal

author email corresponding author email

BMC Genomics 2009, 10:195doi:10.1186/1471-2164-10-195


Published:	27 April 2009

Additional files

Additional file 1:

Data pipeline analysis. Scheme representing the data pipeline used to analyze pyrosequencing data. (A) includes miRDeep analysis and (B) further processing of miRDeep discarded reads.

Format: PDF Size: 92KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

List of known miRNAs and miRNA*. List of known miRNAs and miRNA* identified in this study and its distribution in the small RNA libraries.

Format: XLS Size: 95KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Number of miRNA reads and unique miRNAs throughout development (A) and mature tissues (B). Correlation between the numbers of miRNA reads for each tagged sample and the number of unique miRNAs of each sample.

Format: DOC Size: 36KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 4:

Putative novel miRNAs. List of the novel miRNAs identified, together with information about genomic location, conservation, expression, number of reads and presence or absence of a star sequence.

Format: DOC Size: 68KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 5:

Targets for novel miRNAs. List of the genes that are targets of the novel miRNAs and their classification by GO terms.

Format: DOC Size: 107KB Download file

This file can be viewed with: Microsoft Word Viewer