Research article
Parallel DNA pyrosequencing unveils new zebrafish microRNAs
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* Corresponding author: Manuel AS Santos msantos@ua.pt
1 RNA Biology Laboratory, Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal
2 Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal
3 Biocant Research Centre, 3060-197 Cantanhede, Portugal
4 IEETA, University of Aveiro, 3810-193 Aveiro, Portugal
BMC Genomics 2009, 10:195 doi:10.1186/1471-2164-10-195
Published: 27 April 2009Additional files
Additional file 1:
Data pipeline analysis. Scheme representing the data pipeline used to analyze pyrosequencing data. (A) includes miRDeep analysis and (B) further processing of miRDeep discarded reads.
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Additional file 2:
List of known miRNAs and miRNA*. List of known miRNAs and miRNA* identified in this study and its distribution in the small RNA libraries.
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Additional file 3:
Number of miRNA reads and unique miRNAs throughout development (A) and mature tissues (B). Correlation between the numbers of miRNA reads for each tagged sample and the number of unique miRNAs of each sample.
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Additional file 4:
Putative novel miRNAs. List of the novel miRNAs identified, together with information about genomic location, conservation, expression, number of reads and presence or absence of a star sequence.
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Additional file 5:
Targets for novel miRNAs. List of the genes that are targets of the novel miRNAs and their classification by GO terms.
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