Southern blot analysis of bovine genomic DNA. Genomic DNA from a Bos taurus animal digested with (A) HindIII or (B) Ssp1 was hybridised with probes specific for TRBV9 (lane 1), TRBV6 (lane 2), TRBV20 (lane 3) and TRBV27 (lane 4). (C) Comparison of the banding patters obtained from genomic DNA of a Bos taurus (Bt) and a Bos indicus (Bi) animal hybridised with a probe specific for TRBV9 (lanes 1 and 2) after digestion with HindIII and a probe specific for TRBV27 after digestion with Ssp1 (lanes 3 and 4). Arrows indicate bands that are evident in Bos taurus but not Bos indicus DNA or vice versa.
Connelley et al. BMC Genomics 2009 10:192 doi:10.1186/1471-2164-10-192