Real Time PCR gene expression analysis in bovine donor cells. Gene expression analysis of PALLD, NFYA, GATM and Taspase1 in donor cells lines derived from 0 rounds of cloning (DC0) first round of cloning (DC1), second round of cloning (DC2), fourth round of cloning (DC4), and fifth round of cloning (DC5). Units indicate relative expression to the internal standards GAPDH and 18S rRNA. Different letters indicate significant differences in expression between different donor cell lines (P < 0.01).
Rodriguez-Osorio et al. BMC Genomics 2009 10:190 doi:10.1186/1471-2164-10-190