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Novel transcripts discovered by mining genomic DNA from defined regions of bovine chromosome 6

Rosemarie Weikard*, Tom Goldammer, Annett Eberlein and Christa Kuehn

Author Affiliations

Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), 19196, Dummerstorf, Germany

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BMC Genomics 2009, 10:186  doi:10.1186/1471-2164-10-186

Published: 24 April 2009

Additional files

Additional file 1:

Unique putative exon trapped sequences (ETS) from BAC clones mapped to bovine chromosome 6 (BTA6). The data provided represent BTA6 markers used for BAC library screening, BAC clones subjected to exon trapping and details of the isolated ETS (e.g., accession numbers, primers). Locus: region-specific sequence (markers) used for BAC library screening.

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Additional file 2:

Sequence similarity of identified unique exon trapping sequences (ETS) specific to bovine chromosome 6 (BTA6). Genomic positions on BTA6 and insert sizes of BAC clones were determined according to the bovine genome assembly Btau4.0 at NCBI by in silico screening of BAC end sequences as described previously [9]. Chromosomal positions of ETS were determined by in silico sequence similarity screening to the bovine genome sequence database and chromosome alignment to the bovine genome assembly Btau4.0 at NCBI [31,61]. Comparative sequence similarity of ETS was determined by screening across sequence resources of the human and mouse genomes [62,63]. Similarity to ESTs from Ovis aries and Sus scrofa and to predicted Bos taurus gene models were inferred from the NCBI MapViewer [31] including the additional map options rnaOar, rnaSsc and ab initio. Column 5: UN: unassigned. Column 7: Altern. assembly: human genome assembly based on Celera assembly.

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