Open Access Research article

A high resolution RH map of the bovine major histocompatibility complex

Candice L Brinkmeyer-Langford1, Christopher P Childers2, Krista L Fritz1, Ashley L Gustafson-Seabury1, Marian Cothran1, Terje Raudsepp1, James E Womack3 and Loren C Skow1*

Author Affiliations

1 Department of Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4458, USA

2 Department of Biology, Georgetown University, Washington, DC 20057, USA

3 Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA

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BMC Genomics 2009, 10:182  doi:10.1186/1471-2164-10-182

Published: 24 April 2009

Additional files

Additional file 1:

Primer information. Detailed primer information for RH-mapped markers, including classification (I, IIa, IIb, III), position within Btau_3.1, primer sequences, product sizes, and annealing temperatures used. All PCR reactions were carried out using 1.5 mM MgCl2. *For DQA and DQB two forward primers were multiplexed and used with a single reverse primer.

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Additional file 2:

Marker information. Centiray position, lod score, and frame/placed status for each marker. Note that markers labeled as "frame" are considered to be part of the MLE map. Markers 48.10 and 55.30 could not be assigned a discrete location and instead were tentatively localized to specific regions between two markers as indicated by the column labeled "binned between".

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