Research article
Whole genome amplification and real-time PCR in forensic casework
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* Corresponding author: Emiliano Giardina emiliano.giardina@uniroma2.it
- † Equal contributors
1 Centre of Excellence for Genomic Risk Assessment in Multifactorial and Complex Diseases, School of Medicine, Tor Vergata University of Rome, Rome, Italy
2 Direzione Centrale Anticrimine, Servizio di Polizia Scientifica, Rome, Italy
3 Department of Public Health – Institute of Forensic Medicine, Faculty of Medicine, Tor Vergata University of Rome, Rome, Italy
4 Division of Cardiovascular Medicine, Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
BMC Genomics 2009, 10:159 doi:10.1186/1471-2164-10-159
Published: 14 April 2009Additional files
Additional file 1:
Values of calls, concordant genotypes, concordance rate, call rate and genotype concordance in genomic and amplified DNA with TaqMan® Universal Master Mix. The genotyping results for each SNP are given for dilutions of 1 ng, 0.1 ng and 0.01 ng (first, second and third row respectively). Genotypes derived from direct sequencing were used as reference for determining concordance.
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Additional file 2:
Values of calls, concordant genotypes, concordance rate, call rate and genotype concordance in genomic and amplified DNA with TaqMan® Genotyping Master Mix. The genotyping results for each SNP are given for dilutions of 1 ng, 0.1 ng and 0.01 ng (first, second and third row respectively). Genotypes derived from direct sequencing were used as reference for determining concordance.
Format: DOC Size: 161KB Download file
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Additional file 3:
Values of calls, concordant genotypes, concordance rate, call rate and genotype concordance in genomic and amplified artificially-degraded DNA. The results reported are obtained from MDA amplification of high artificially-degraded DNA (180 s). Genotypes derived from direct sequencing were used as reference for determining concordance.
Format: DOC Size: 74KB Download file
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