Research article
Chromosome Y variants from different inbred mouse strains are linked to differences in the morphologic and molecular responses of cardiac cells to postpubertal testosterone
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* Corresponding author: Christian F Deschepper deschec@ircm.qc.ca
- † Equal contributors
1 Experimental Cardiovascular Biology Research Unit, Institut de recherches cliniques de Montréal (IRCM) and Université de Montréal, 110 Pine Ave West, Montréal (QC), H2W 1R7, Canada
2 The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
3 Present address: Australian Centre for Ancient DNA, University of Adelaide, SA 5005, Australia
BMC Genomics 2009, 10:150 doi:10.1186/1471-2164-10-150
Published: 7 April 2009Additional files
Additional file 1:
List of 249 interaction genes. List of 249 interaction genes (with significant interaction between the effects of strain and surgery). Fold change (FC) was calculated as the ratio of mean expression of either: 1) C57BL/6J over C57.YA mice (Strain); or 2) Castrated over sham-operated (Treatment) mice. Ratios below 1 are expressed as the negative of the inverse ratio (e.g. a ratio of 0.75 is equivalent to a -1.3 FC). FC equal or higher than 1.5 are formatted in bold. For genes represented by multiple probes in the microarray, only the probe with the lowest p-value for the interaction term is included. P-values for the interaction between strain and treatment effects are adjusted for multiple comparisons with an FDR transformation (see methods).
Format: XLS Size: 72KB Download file
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Additional file 2:
Differential expression of genes in selected KEGG pathways. Differential expression of genes in selected KEGG pathways. Fold change (FC) was calculated as the ratio of mean expression in CX vs. sham-operated group. Ratios below 1 are expressed as the negative of the inverse ratio (e.g. a ratio of 0.75 is equivalent to a -1.3 FC). P-values for the interaction between strain and treatment effects are adjusted for multiple comparisons with an FDR transformation (see methods). P-values less than 0.1 are in bold. For genes represented by multiple probes in the microarray, only the probe with the lowest p-value for the interaction term is included. Genes are sorted by p-values within pathways.
Format: XLS Size: 70KB Download file
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Additional file 3:
Primers used for RT-PCR amplification of chr Y mRNA transcripts. Primers used for RT-PCR amplification of chr Y mRNA transcripts.
Format: DOC Size: 78KB Download file
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