Figure 4.

Functional validation of precursors MG008-X and MG053-01. Detection of miRNAs MG008 and MG053 after transfection of COS-7 cell with expression vectors vMG008 and vMG053 by Northern blot analysis. RNAs were extracted from cells transfected with expression vectors (T) and with the control empty vector (C), separated onto a 15% denaturing PAGE and transferred onto a nylon membrane Blots were hybridized with miRNA antisense oligonucleotides and a U6 probe used as an internal loading control.