Additional file 1.dPCR analysis of mock library control. The figure shows the absence of digital counts from a mock sequencing library preparation (454). False-color image of 12.765 digital array at assay endpoint. Each grid point corresponds to a nanoliter-scale PCR reaction, with yellow squares revealing amplification due to the presence of at least one sequencing library template molecule. The panels show dilution series (indicated) of a library preparation carried out as usual but for omission of sample DNA. The Ace sample (described in Table 2 of the main text) is used here as a positive control. Format: PDF Size: 7.4MB Download file This file can be viewed with: Adobe Acrobat Reader White et al. BMC Genomics 2009 10:116 doi:10.1186/1471-2164-10-116 |