Overexpression of Scg5 increases enzymatic activity of PCSK2 and is inversely correlated with body weight in congenic mice

Charles R Farber¹^,3 , James Chitwood¹ , Sang-Nam Lee²^,4 , Ricardo A Verdugo¹ , Alma Islas-Trejo¹ , Gonzalo Rincon¹ , Iris Lindberg²^,5 and Juan F Medrano¹

¹Department of Animal Science, University of California, Davis, One Shields Ave., Davis, CA 95616-8521, USA

²Department of Biochemistry and Molecular Biology, Louisiana State University Health, Sciences Center, and Children's Hospital Research Institute, New Orleans, LA 70112-2223, USA

³Department of Medicine, Division of Cardiology, University of, California, Los Angeles, 695 Charles E. Young Drive South, Los Angeles, CA 90095-1679, USA

⁴Research Center for Human Natural Defense System, Yonsei, University College of Medicine, Seoul, 120-752, Korea

⁵Department of Anatomy and Neurobiology, University of, Maryland Medical School, 20 Penn St, Baltimore, MS 21201, USA

author email corresponding author email

BMC Genetics 2008, 9:34doi:10.1186/1471-2156-9-34


Published:	25 April 2008

Additional files

Additional file 1:

Differentially expressed genes in HG2DF2 non-recombinant F2 mice. These data represent a list of genes differentially expressed in HG2DF2 non-recombinant F2 mice determined using DNA microarrays.

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Additional file 2:

Scg5 whole brain expression is up-regulated in HG versus B6 male mice at 3, 4.5 and 9 weeks of age and the Scg5 promoter contains two tandem putative Stat5b DNA binding sites. Expression analysis of Scg5 as a function of HG genotype.

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Additional file 3:

Scg5 haplotypes based on available SNP data. Haplotype analysis of Scg5 in "low" and "high" expressing strains using publically available SNP data.

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Additional file 4:

Hierarchical clustering analysis of Scg5 haplotype blocks. Illustrates the relationships of Scg5 haplotype blocks in "low" and "high" expressing strains.

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Additional file 5:

Scg5 promoter sequencing primers. List of PCR primers used for Scg5 sequencing.

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