Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

doi:10.1186/1471-2156-8-61

BMC Genetics

Volume 8

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Research article

Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

James A Coker and Shiladitya DasSarma

University of Maryland Biotechnology Institute, Center of Marine Biotechnology, 701 East Pratt Street, Baltimore, MD 21202, USA

author email corresponding author email

BMC Genetics 2007, 8:61doi:10.1186/1471-2156-8-61


Published:	24 September 2007

Additional files

Additional file 1:

Average values for all genes in the two ΔtbpD microarrays and two dye swapped ΔtbpD microarrays. Average values for log₂(x), standard deviation of log₂(x), fold changes, and p value for the two ΔtbpD and the two ΔtbpD dye swap arrays.

Format: XLS Size: 561KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Average values for all genes in the two ΔtfbA microarrays and two dye swapped ΔtfbA microarrays. Average values for log₂(x), standard deviation of log₂(x), fold changes, and p value for the two ΔtfbA and the two ΔtfbA dye swap arrays.

Format: XLS Size: 559KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

List of primers used in creating the first constructs. List of primers used to amplify the tbp/tfb genes and their surrounding area to create the first constructs and screen for knockouts.

Format: PDF Size: 69KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 4:

List of primers used in creating the knockout constructs. List of primers used to amplify most of the first constructs to create the tbp/tfb knockout constructs used in this study.

Format: PDF Size: 71KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 5:

List of the microarray data of the heat shock response genes from Halobacterium sp. NRC-1. Average values for log₂(x), standard deviation of log₂(x), fold changes, and p value for the heat shock gene transcripts in the two ΔtbpD and the two ΔtbpD dye swap arrays and the two ΔtfbA and the two ΔtfbA dye swap arrays.

Format: XLS Size: 19KB Download file

This file can be viewed with: Microsoft Excel Viewer