Table 1


Primers	Forward 5'-3'	Reverse 5'-3'	TM

1	atgcaacagggagaaagagc	gaccagcattggtagcaggt	57°C
2	tggacagcacggctctaaat	tggacagcacggctctaaat	58°C
3	ggtctggtgatgacagttgtg	ggaagggaggcatgtcagta	60°C
4	tgtattttagtacagacggggtttc	tggtgcctgattatgcagag	60°C
5	gcttagcagtgcctgtccat	gccaaattgctgcctattct	58°C
6	agggtggaagatctcagaag	caaagcgctgggattatg	57°C
7	gctgaggcaggagaatcact	ttggccacccaacagttaat	59°C
8	aaataaaaaggaagaagaagaaga	ccacccaacagttaataccta	57°C
9	ggcagcaatggaattgaaag	cccacagtgctgggattac	58°C
10	ccaggggtttgcagttacag	gcttattcactaggaccaagcac	57°C
11	ttgcagtgagccaaaatcc	tcacccatcatgcaaaacat	63°C
12	tgacctcaggtgatccactc	ggtgaaactgagggagtttg	62°C
13	tgagaccctctccagtttgc	agacgaagaggtgggaacag	60°C
14	tcacatcttacaaactcaacaaaaa	ccaggagggttgcttaggta	59°C
15	ccagtctggcctcgagaact	tcacaaatattggccaggtg	59°C
16	ccagtcctgtaggtgctaaa	cttagccctgaaggaaaaat	56°C
17	tctaaatcactagcatttcctg	gaaagcattccgtaagatgt	58°C
18	tgggttcagaagttcttctcacta	ccccataccctccttgctac	62°C
19	gccagagcaggaagagtc	cgttgccactttgtttatc	56°C
20	gctactcactaaatgttcagctcct	cttgttcccccaggtgact	62°C
21	ctccaccggacttttggtaa	ggtgctcttctgttgccatt	57°C
22	gaattgacccctcaaggaca	aggcagaggtttcagtgagc	61°C
23	ggcagagttttgctcttgtca	tggtgaaaccccgtctctac	58°C
24	agtgcaggggcaggatct	gcatggggacagaggaataa	58°C
25	ccttttctcttcattttcccatt	ggatgagaggaaggaaagattc	59°C
26	tgcctgctttagaatgttttcc	agacaggtgcgtggataagg	59°C
27	aagttttcataagcacacttctaacc	tctctccagacaccagaagg	61°C

Marrosu et al. BMC Genetics 2007 8:25 doi:10.1186/1471-2156-8-25