Figure 3.

Comparison of standard PCR with hemi-nested touchdown (TD) PCR. (A) Standard PCR with primers LMWF38d/R19b and annealing temperature of 50°C, (B) the first round TD PCR using primers LMWF38d/R19b and (C) the second round hemi-nested TD PCR using primers LMWF38d/R19c. In the TD program, the annealing temperature is progressively lowered from 65°C to 50°C by 0.5°C every cycle, followed by 15 additional cycles at 50°C. M, 1 kb plus DNA ladder; lanes 1–7, PCR amplification of TaE0879B17, TaE0877F13, TaE0742B23, TaE0469B11, TaE0428D3, TaE0356P04 and TaE0212J01, respectively.