Figure 1.

Amplification of eight representative BAC clones with primer pair LMWF3/LMWR4 shows variable fragment sizes. Amplicon intensity represents various primer-template mismatches. M, 1 kb plus DNA ladder; lanes 1–8, PCR amplification of TaE0503E07, TaE1238L16, TaE0154F22, TaE0359D24, TaE01157C20, TaE0709A09, TaE0099E23 and TaE0072P19, respectively.