Figure 7.

CDA-related genes according to their function. A) Alignment of amino acid sequences of maize cytidine deaminase and B. subtilis nucleoside deaminase. Identical and conserved amino acids are marked by asterisks and dots, respectively; hyphens represent gaps. B) Alignment of the active sites of cytidine deaminases. Accession numbers of amino acid sequences used here are as follows: [GenBank: APOBEC3, Q9HC16; AID, Q9GZX7]; mouse [GenBank: APOBEC1, U21951]; rat [GenBank: APOBEC1, L07114]; human [GenBank: APOBEC1, L25877]; rabbit [GenBank: APOBEC1, U10695]; B. subtilis cytidine deaminase [GenBank: P19079]; human cytidine deaminase [GenBank: L27943]; mouse cytidine deaminase [GenBank: P56389]; E. coli cytidine deaminase [GenBank: P13652]; H. influenzae cytidine deaminase [GenBank: U32814]; Arabidopsis cytidine deaminase [GenBank: AJ005687]; rice cytidine deaminase [GenBank: NP_916608]; B. cereus nucleoside deaminase [GenBank: NP_829925]; B. cereus nucleoside deaminase [GenBank: AAP07126]; B. fragilis nucleoside deaminase [GenBank: BAD48383]; B. subtilis nucleoside deaminase [GenBank: O34598]; Arabidopsis nucleoside deaminase [GenBank: NP_198157]; maize nucleoside deaminase, this work [GenBank: EF106973]; rice nucleoside deaminase [GenBank: XP_470319]; S. cerevisiae deoxycytidylate deaminase [GenBank: P06773]; T2/T4 phage deoxycytidylate deaminase [GenBank: P00814]; human deoxycytidylate deaminase [GenBank: L12136]; mouse deoxycytidylate deaminase [GenBank: Q8K2D6]; Arabidopsis deoxycytidylate deaminase [GenBank: ABE66000]; rice deoxycytidylate deaminase [GenBank: BAD87146]. C) The phylogenetic tree was constructed based on the active site of cytidine deaminases using the PAUP 4.0b10 UPGMA method [50]. Numbers indicate bootstrap values (above 50% with 1000 replications).