Figure 6.

Multinucleate cells in mutant clones. (A-F) Mutations leading to cytokinesis or membrane defects in MARCM clones. DNA staining (blue); Miranda (red); CD8::GFP (green); Bar: 50 μm (A), 10 μm (B-F). The C33 mutation, which maps to the pebble locus, result in a dramatic increase in cell size and DNA content and clones can occupy a substantial proportion of the optic lobe (A). Clones of the J2 (B), D24 (C) or GL45 (D) chromosomes also give rise to multinucleated cells which typically contain only two nuclei. Mutation of O29 (E) or CMV45 (F) leads to the formation of punctae labelled with CD8::GFP (arrows) or an excess of GFP staining; the origin of both these phenotypes is unclear.