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Table 6
*PCR Primers used for the MLPH* cDNA amplification and/or genomic MLPH mutation analysis**
primer name	primer sequence	product size	T_M	comments

MLPH cDNA, first round amplification
Mlph_cDNA_Ex1_F1	CCT TCC TTC CCC TGT AGG AC	513 bp	52°C
Mlph_cDNA_Ex4_R	GGA TCA CCT TGG CAC TCC
Mlph_cDNA_Ex4_F1	GTG AAG ATC GGC TCG GTA G	686 bp	52°C
Mlph_cDNA_Ex9_R	GGA TGC TGA GAG GTG GTG
Mlph_cDNA_Ex7_F1	CTT GGA CTT TGA GGC AGA C	985 bp	52°C
Mlph_cDNA_Ex14_R	ACT GAA CTT CCT TCT GAG G
Mlph_cDNA_Ex10_F	AGA GAA GAG GAG ACC CTC	651 bp	52°C
Mlph_cDNA_Ex16_R	GCT GGG TCA TCA CAG GC

MLPH cDNA, second round amplification
Mlph_cDNA_Ex1_F2	CTG TAG GAC CGG AGA GAG C	502 bp	52°C
Mlph_cDNA_Ex4_R	GGA TCA CCT TGG CAC TCC
Mlph_cDNA_Ex4_F2	AGA TCG GCT CGG TAG AGT G	679 bp	52°C
Mlph_cDNA_Ex9_R	GGA TGC TGA GAG GTG GTG
Mlph_cDNA_Ex7_F2	CTC TGA CGA CTC CAC TTG	967 bp	52°C
Mlph_cDNA_Ex14_R	ACT GAA CTT CCT TCT GAG G
Mlph_cDNA_Ex10_F	AGA GAA GAG GAG ACC CTC	651 bp	52°C	same primers as in first round amplification
Mlph_cDNA_Ex16_R	GCT GGG TCA TCA CAG GC

MLPH genomic DNA
P_Mlph_Ex1_for1	AGT GGC CTC AAG CCC TG	591 bp	56°C
P_Mlph_Ex1_rev	ATG AGC TCC CTG AGA ACC
P_Mlph_Ex1_for2	CTC CTC CCG AGG CTC CTG	563 bp	56°C
P_Mlph_Ex1_rev	ATG AGC TCC CTG AGA ACC
Mlph_Ex2_for	GTC ACC GAC ATT ATG TCA CAG	598 bp	58°C
Mlph_Ex2_rev	CAG GCT GGA AGG TCA GAT C
Mlph_Ex3&4_for	GAG ACC CAG GAT CGA GTC	725 bp	56°C
Mlph_Ex3&4_rev	CAC TCA CAT TCC AAA GGA TC
Mlph_Ex5_for	AGA AGT GAT GGA GGT CAC TG	471 bp	56°C
Mlph_Ex5_rev	ACC GAA CAG GAA CAG GAG
Mlph_Ex6_for	AG CTT GCC TGG ATG GAT G	582 bp	53°C
Mlph_Ex6_rev	CTG CAG CCT CTG CCA AC
Mlph_Ex7_for	GTC CTC AGC ACT TCT GAG	568 bp	53°C
Mlph_Ex7_rev	GTG AGA AGC TTC TGG ACC
Mlph_Ex8_for	CAG CGG GAT TTC TGA AAG C	483 bp	56°C
Mlph_Ex8_rev	GTG TTG GAC AGT CAG AGT G
Mlph_Ex9_for	CCC GCC TTT GCC TTA AGC	416 bp	56°C
Mlph_Ex9_rev	GCT GCA AGG AGG AGC TTC
Mlph_Ex10_for	CAG AGC CTG GCT CCT GAG	600 bp	56°C
Mlph_Ex10_rev	CAC CTG GGA CAG GGA AGC
Mlph_Ex11_for	AGG CGT CCA GAG GTT GTG	550 bp	55°C
Mlph_Ex11_rev	GCC TGC TTC TGG AGA AGC
Mlph_Ex12_for	CGC CGA CCA AGT CTT TGC	623 bp	55°C
Mlph_Ex12_rev	TGG ACT TGA GGC CGT GTG
Mlph_Ex13_for	CCT GTC TCC CCA GAT TCG	539 bp	55°C
Mlph_Ex13_rev	GGG TTT GCC AAG GCT GAG
Mlph_Ex14_for	CTC CTT TAT GCT CTG GCA C	591 bp	55°C
Mlph_Ex14_rev	TTG TCA CAC GGA GAC ACA G
Mlph_Ex15_for	CTC AGG CAG GCA GAC AAG	461 bp	55°C
Mlph_Ex15_rev	TGC TCT GGG GTC CTA ACG
P_MLph_Ex16_for	GCT TCA GAG CCT GAA ATT CT	469 bp	53°C
P_Mlph_Ex16_rev	GAC AAA AGA TCA GGC TGG AG

Philipp et al. BMC Genetics 2005 6:34 doi:10.1186/1471-2156-6-34

Table 6