Table 1

HAP-sensitivity of the mutants selected in our screening.

Class of mutants

Gene or ORF name deleted

Functional group

Response HAP in spot tests

Induced mutant frequency (×10-7) by HAP#

Survival in presence of HAP#


Wild type

-

400

100%


Class I: mutants hypersensitive to HAP

HAM1

DNA1

HM, HS

13000

17%

ADE12*

DNA

HM, HS

10000

30%

AAH1*

DNA

M, S

5500

30%

ADE2

DNA

M, S

4400

70%


II class: Mutants sensitive to mutagenic effect of HAP

VIP1

Cell2

M, S

840

76%

VID27

Metabolic3

M, S

600°

82%

IPK1

Metabolic

M, S

1400

100%

ADE5,7

DNA

M

1900

100%

ADE8

DNA

M

1500

100%

ADE6

DNA

M

1100

100%

RIM101

meiosis

M

1100

100%

ADE3

DNA

M

940

100%

ADE1

DNA

M

860

100%

YGR035c

unknown

M

500°

100%

Yjl055w*

Metabolic

M

500°

100%


Class III: Mutants sensitive to killing

YMl013c-a

Unknown

S

230°

60%

SHE4

Cell

S

600°

30%

TRP2

Metabolic

HS

250°

60%


†- HM – hypermutable, M – more mutable than wild-type, HS – hypersensitive, S – more sensitive than wild-type, see first paragraph od the Results Section for the explanation.

# – 25 μg/ml

1 – 'DNA' – includes genes involved in the control of DNA precursor metabolism, purine salvage, DNA repair.

2 – 'Cell' – includes genes involved in cytoskeleton organization, cell walls and organelles.

3 – 'Metabolic' – includes genes involved in general metabolic pathways.

* – These mutants were also sensitive to the mutagenic or toxic effect of AHA (see Fig. 3).

° – small difference from the wild-type strain was reproducible and statistically significant by Wilcoxon-Mann-Whitney test.

Stepchenkova et al. BMC Genetics 2005 6:31   doi:10.1186/1471-2156-6-31

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