Table 1

Ts mutant frequency among: Ade+ revertants, Ade- survivors and colonies from the log phase. The frequency of ts mutants in three independent experiments was estimated using a random sample of de3-01-CG Ade+ revertants, Ade- survivors isolated on day 14 and Ade+ revertants obtained from exponentially growing cells. A hundred Ade+ revertants of the strain CG379-3-29(LR) starved for adenine were used as controls. The percentage of ts mutants with respect to the number of tested colonies is reported in parentheses.

Revertants/Survivors
No. of tested colonies
No. of ts mutants

Starvation condition day 14
de3-01-CG Ade+
489
249 (51%)
de3-01-CG Ade-
151
59 (39%)
CG379-3-29(LR) Ade+
100
0
Log phase cells
de3-01-CG Ade+
1070
7a

a The percentage has not been calculated since the ts mutants are likely to be a single clone.

Achilli et al. BMC Genetics 2004 5:34   doi:10.1186/1471-2156-5-34