|
Ts mutant frequency among: Ade+ revertants, Ade- survivors and colonies from the log phase. The frequency of ts mutants in three independent experiments was estimated using a random sample of de3-01-CG Ade+ revertants, Ade- survivors isolated on day 14 and Ade+ revertants obtained from exponentially growing cells. A hundred Ade+ revertants of the strain CG379-3-29(LR) starved for adenine were used as controls. The percentage of ts mutants with respect to the number of tested colonies is reported in parentheses. |
||
| Revertants/Survivors |
No. of tested colonies |
No. of ts mutants |
|
|
||
| Starvation condition day 14 |
||
| de3-01-CG Ade+ |
489 |
249 (51%) |
| de3-01-CG Ade- |
151 |
59 (39%) |
| CG379-3-29(LR) Ade+ |
100 |
0 |
| Log phase cells |
||
| de3-01-CG Ade+ |
1070 |
7a |
|
a The percentage has not been calculated since the ts mutants are likely to be a single clone. | ||
Achilli et al. BMC Genetics 2004 5:34 doi:10.1186/1471-2156-5-34 |
||