Table 2 |
||||
|
Oligonucleotide pairs used in the amplification and sequencing |
||||
|
Fragment |
Annealing |
|||
|
Name |
CRS reference |
Sequence (5'–3') |
size (pb) |
temp.(°C) |
|
|
||||
|
L16340 |
(16318–16340) |
AGCCATTTACCGTACATAGCACA |
681 |
52 |
|
H408 |
(429–408) |
TGTTAAAAGTGCATACCGCCA |
||
|
L382 |
(362–382) |
CAAAGAACCCTAACACCAGCC |
603 |
56 |
|
H945 |
(964–945) |
GGGAGGGGGTGATCTAAAAC |
||
|
L923 |
(902–923) |
GTCACACGATTAACCCAAGTCA |
607 |
56 |
|
H1487 |
(1508–1487) |
GTATACTTGAGGAGGGTGACGG |
||
|
L1466 |
(1445–1466) |
GAGTGCTTAGTTGAACAGGGCC |
629 |
58 |
|
H2053 |
(2073–2053) |
TTAGAGGGTTCTGTGGGCAAA |
||
|
L2025 |
(2004–2025) |
GCCTGGTGATAGCTGGTTGTCC |
609 |
52 |
|
H2591 |
(2612–2591) |
GGAACAAGTGATTATGCTACCT |
||
|
L2559 |
(2538–2559) |
CACCGCCTGCCCAGTGACACAT |
591 |
56 |
|
H3108 |
(3128–3108) |
TCGTACAGGGAGGAATTTGAA |
||
|
L3073 |
(3051–3073) |
AAAGTCCTACGTGATCTGAGTTC |
640 |
52 |
|
H3670 |
(3690–3670) |
GGCGTAGTTTGAGTTTGATGC |
||
|
L3644 |
(3625–3644) |
GCCACCTCTAGCCTAGCCGT |
623 |
58 |
|
H4227 |
(4247–4227) |
ATGCTGGAGATTGTAATGGGT |
||
|
L4210 |
(4189–4210) |
CCACTCACCCTAGCATTACTTA |
625 |
55 |
|
H4792 |
(4813–4792) |
ACTCAGAAGTGAAAGGGGGCTA |
||
|
L4750 |
(4729–4750) |
CCAATACTACCAATCAATACTC |
599 |
52 |
|
H5306 |
(5327–5306) |
GGTGATGGTGGCTATGATGGTG |
||
|
L5278 |
(5259–5278) |
TGGGCCATTATCGAAGAATT |
593 |
58 |
|
H5832 |
(5851–5832) |
GACAGGGGTTAGGCCTCTTT |
||
|
L5781 |
(5762–5781) |
AGCCCCGGCAGGTTTGAAGC |
626 |
58 |
|
H6367 |
(6387–6367) |
TGGCCCCTAAGATAGAGGAGA |
||
|
L6337 |
(6318–6337) |
CCTGGAGCCTCCGTAGACCT |
601 |
58 |
|
H6899 |
(6918–6899) |
GCACTGCAGCAGATCATTTC |
||
|
L6869 |
(6850–6869) |
CCGGCGTCAAAGTATTTAGC |
578 |
58 |
|
H7406 |
(7427–7406) |
GGGTTCTTCGAATGTGTGGTAG |
||
|
L7379 |
(7358–7379) |
AGAAGAACCCTCCATAAACCTG |
580 |
56 |
|
H7918 |
(7937–7918) |
AGATTAGTCCGCCGTAGTCG |
||
|
L7882 |
(7861–7882) |
TCCCTCCCTTACCATCAAATCA |
506 |
56 |
|
H8345 |
(8366–8345) |
TTTCACTGTAAAGAGGTGTTGG |
||
|
L8299 |
(8280–8299) |
ACCCCCTCTAGAGCCCACTG |
603 |
56 |
|
H8861 |
(8882–8861) |
GAGCGAAAGCCTATAATCACTG |
||
|
L8799 |
(8779–8799) |
CTCGGACTCCTGCCTCACTCA |
638 |
58 |
|
H9397 |
(9416–9397) |
GTGGCCTTGGTATGTGCTTT |
||
|
L9362 |
(9342–9362) |
GGCCTACTAACCAACACACTA |
609 |
56 |
|
H9928 |
(9950–9928) |
AACCACATCTACAAAATGCCAGT |
||
|
L9886 |
(9865–9886) |
TCCGCCAACTAATATTTCACTT |
617 |
56 |
|
H10462 |
(10481–10462) |
AATGAGGGGCATTTGGTAAA |
||
|
L10403 |
(10383–10403) |
AAAGGATTAGACTGAACCGAA |
612 |
56 |
|
H10975 |
(10994–10975) |
CCATGATTGTGAGGGGTAGG |
||
|
L10949 |
(10930–10949) |
CTCCGACCCCCTAACAACCC |
617 |
58 |
|
H11527 |
(11546–11527 |
CAAGGAAGGGGTAGGCTATG |
||
|
L11486 |
(11467–11486 |
AAAACTAGGCGGCTATGGTA |
629 |
56 |
|
H12076 |
(12095–12076 |
GGAGAATGGGGGATAGGTGT |
||
|
L12028 |
(12008–12028 |
GGCTCACTCACCCACCACATT |
615 |
58 |
|
H12603 |
(12623–12603 |
ACGAACAATGCTACAGGGATG |
||
|
L12572 |
(12553–12572 |
ACAACCCAGCTCTCCCTAAG |
591 |
56 |
|
H13124 |
(13143–13124 |
ATTTTCTGCTAGGGGGTGGA |
||
|
L13088 |
(13068–13088 |
AGCCCTACTCCACTCAAGCAC |
618 |
58 |
|
H13666 |
(13685–13666 |
AGGGTGGGGTTATTTTCGTT |
||
|
L13612 |
(13593–13612 |
AAGCGCCTATAGCACTCGAA |
614 |
56 |
|
H14186 |
(14206–14186 |
TGGTTGAACATTGTTTGTTGG |
||
|
L13612 |
(13593–13612 |
AAGCGCCTATAGCACTCGAA |
614 |
56 |
|
H14186 |
(14206–14186 |
TGGTTGAACATTGTTTGTTGG |
||
|
L14125 |
(14104–14125 |
TCTTTCTTCTTCCCACTCATCC |
602 |
58 |
|
H14685 |
(14705–14685 |
CATTGGTCGTGGTTGTAGTCC |
||
|
L14650 |
(14629–14650 |
CCCCATTACTAAACCCACACTC |
604 |
58 |
|
H15211 |
(15232–15211 |
TTGAACTAGGTCTGTCCCAATG |
||
|
L15162 |
(15143–15162 |
CTCCCGTGAGGCCAAATATC |
597 |
58 |
|
H15720 |
(15739–15720 |
GTCTGCGGCTAGGAGTCAAT |
||
|
L15676 |
(15657–15676 |
TCCCCATCCTCCATATATCC |
524 |
56 |
|
H16157 |
(16180–16157 |
TGATGTGGATTGGGTTTTTATGTA |
||
|
L15996 |
(15975–15996 |
CTCCACCATTAGCACCCAAAGC |
446 |
58 |
|
H16401 |
(16420–16401 |
TGATTTCACGGAGGATGGTG |
||
|
|
||||
|
Maca-Meyer et al. BMC Genetics 2001 2:13 doi:10.1186/1471-2156-2-13 |
||||
