Table 3

Expected rates of false positive results and expected positive predictive values for differentRHD PCR strategies*

Rate of

Positive predictive value

Number of

PCR strategy

false positives

of positive result

polymorphism tested


Exon 10 only [1,2]

1:1,276

0.999216

1

Intron 4/Exon 7 [13]

1:4,081

0.999755

2

Intron 4/Exon 7/RHDψ [14]

1:4,700

0.999787

3

Intron 4/Exon 7/W16X

1:5,212

0.999808

3

Intron 4/Exon 7/Intron 7

1:6,051

0.999835

3

Exons 3, 4, 5, 6, 7, 9 [26]

1:6,051

0.999835

6

Exons 2, 3, 4, 5, 6, 7, 9, 10 [27]

1:6,051

0.999835

8

Intron 4/Exon 7/W16X/RHDψ

1:6,267

0.999840

4

All Exons/RHDψ

1:7,520

0.999867

9

Intron 4/Exon 7/Intron 7/W16X

1:8,921

0.999888

4

Intron 4/Exon 7/Intron 7/W16X/RHDψ

1:12,533

0.999920

5


* Rates were calculated based on the population frequencies of different alleles determined in the Table 2. The exact rates are population dependent and may vary according to the prevalence of alleles in the population tested.

Wagner et al. BMC Genetics 2001 2:10   doi:10.1186/1471-2156-2-10

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