Email updates

Keep up to date with the latest news and content from BMC Genetics and BioMed Central.

Open Access Research article

Genotype is an important determinant factor of host susceptibility to periodontitis in the Collaborative Cross and inbred mouse populations

Ariel Shusterman1, Yasser Salyma2, Aysar Nashef1, Morris Soller3, Asaf Wilensky4, Richard Mott5, Ervin I Weiss1, Yael Houri-Haddad1 and Fuad A Iraqi2*

Author Affiliations

1 Department of Prosthodontics, Faculty of Dental Medicine, Hadassah Medical Centers and The Hebrew University, Jerusalem, Israel

2 Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

3 Department of Genetics, Hebrew University, Jerusalem, Israel

4 Department of Periodontology, Faculty of Dental Medicine, Hadassah Medical Centers and The Hebrew University, Jerusalem, Israel

5 Wellcome Trust Human Genome Centre, Oxford University, Oxford OX3 7BN, UK

For all author emails, please log on.

BMC Genetics 2013, 14:68  doi:10.1186/1471-2156-14-68

Published: 9 August 2013

Abstract

Background

Periodontal infection (Periodontitis) is a chronic inflammatory disease, which results in the breakdown of the supporting tissues of the teeth. Previous epidemiological studies have suggested that resistance to chronic periodontitis is controlled to some extent by genetic factors of the host. The aim of this study was to determine the phenotypic response of inbred and Collaborative Cross (CC) mouse populations to periodontal bacterial challenge, using an experimental periodontitis model. In this model, mice are co-infected with Porphyromonas gingivalis and Fusobacterium nucleatum, bacterial strains associated with human periodontal disease. Six weeks following the infection, the maxillary jaws were harvested and analyzed for alveolar bone loss relative to uninfected controls, using computerized microtomography (microCT). Initially, four commercial inbred mouse strains were examined to calibrate the procedure and test for gender effects. Subsequently, we applied the same protocol to 23 lines (at inbreeding generations 10–18) from the newly developed mouse genetic reference population, the Collaborative Cross (CC) to determine heritability and genetic variation of control bone volume prior to infection (CBV, naïve bone volume around the teeth of uninfected mice), and residual bone volume (RBV, bone volume after infection) and loss of bone volume (LBV, the difference between CBV and RBV) following infection.

Results

BALB/CJ mice were highly susceptible (P<0.05) whereas DBA/2J, C57BL/6J and A/J mice were resistant. Six lines of the tested CC population were susceptible, whereas the remaining lines were resistant to alveolar bone loss. Gender effects on bone volume were tested across the four inbred and 23 CC lines, and found not to be significant. Based on ANOVA analyses, broad-sense heritabilities were statistically significant and equal to 0.4 for CBV and 0.2 for LBV.

Conclusions

The moderate heritability values indicate that the variation in host susceptibility to the disease is controlled to an appreciable extent by genetic factors. These results strongly support the possibility of using the Collaborative Cross, as well as developing dedicated F2 (resistant x susceptible inbred strains) resource populations, for future dissection of genetic factors in periodontitis.

Keywords:
Periodontal infection; Experimental periodontitis; microCT; Collaborative cross; Genes; Heritability