Open Access Research article

Carriers of a novel frame-shift insertion in WNT16a possess elevated pancreatic expression of TCF7L2

Eric W Howard1, Latonya F Been2, Megan Lerner3, Daniel Brackett4, Stan Lightfoot3, Elizabeth C Bullen1 and Dharambir K Sanghera2*

Author Affiliations

1 Department of Cell Biology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

2 Department of Pediatrics, College of Medicine, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd., Rm 317 BMSB, Oklahoma City, OK, 73104, USA

3 Department of Surgery, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

4 Veteran Affairs, VA Medical Center, Oklahoma City, OK, USA

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BMC Genetics 2013, 14:28  doi:10.1186/1471-2156-14-28

Published: 23 April 2013

Additional files

Additional file 1: Figure S1:

Exome sequencing reveals the presence of 4 base pair insertion (CCCA) between A and T of ATG start codon in Wnt16a which was confirmed by targeted sequencing of 30 DNA samples using an ABI 3730 sequencer (Applied Biosystemes Inc. Foster City, USA) and were analyzed using Mutation Surveyor (v4.0.6.). Figure S2. Nusieve-Agarose (3:1) gel showing wild-type (458 bp), heterozygous insertion (458/462 bp) and homozygous insertion (462 bp) bands in WNt16a gene. Figure S3. Oligonucleotides used to amplify Wnt-16a luciferase reporter constructs. Figure S4. Comparative genomic analysis showing evolution of translation initiation sites in Wnt16a. Arrow indicates the position of insertion in the evolutionarily conserved region at the start codon.

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Additional file 2: Table S1:

Genome-wide Exome Sequencing in Asian Sikhs.

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