Phylogenetic tree of WC1 domain 1 sequences. The evolutionary history of 90 taxa was inferred using Bayesian analysis in MrBayes3.2 . Annotated sequences were designed with a prefix of “A”. Sequences obtained from individual animal of three breeds (Hereford, Belted Galloway, and Holstein) were designed with prefixes of “D”, “Y”, and “B”, respectively. Genomic DNA and transcripts sequences were designed with prefixes of “G and cDNA”, respectively. Markov chain Monte Carlo analysis was performed for 830,000 cycles, using 2 runs of 4 chains each, a temperature setting of 0.2, and an amino acid mixed model to approximate the posterior probabilities of trees, shown at branch nodes. The average standard deviation of split frequencies was 0.01, which was diagnostic of convergence at <0.05. Bootstrap values >70 are shown.
Chen et al. BMC Genetics 2012 13:86 doi:10.1186/1471-2156-13-86